Background Correction

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• Rolling-ball / top-hat transform: estimates and subtracts a slowly varying background using morphological operations.

• Flat-field correction: divides by a reference image acquired under the same conditions without the sample.

See also

• Morphological Operations — the operations behind the rolling-ball/top-hat transform

• Contrast Enhancement — CLAHE, a complementary way to handle non-uniform illumination

• Image Preprocessing — every other preprocessing operation